Days to Teach:
2-3 43 minute class periods
Teaching Tips / Activity Overview:
This protocol is designed for a 43 minute class period, but could take up to 2-3 43 minute class periods depending upon the development of the gels. Students may need additional time to research the bacteria and primer information before this lab if students are not very familiar with using the research tools or did not have enough time during the previous class period.
- Gel electrophoresis group review of purpose, technique and result (5 minutes)
- Prepare the gels and run the gels for the 3 bacterial samples after the PCR is completed (30 minutes)
- While the gels are running, discuss/summarize the results from research regarding basic characteristics about the 3-4 bacterial samples being used and the 2 primers (20 minutes)
- Analyze the gel results and draw conclusions for the protocol. (5 minutes)