Teaching Tips / Activity Overview:
- Prep Lab: Teacher will discuss lab with students and review proper procedure.
- Electrophoresis Lab: Students take the 1st day to make the agarose, let the gel sit, load the gel, and allow the gel to start to run. The staining the students will probably start, but the teacher will finish after school or throughout the day.
- Analysis: Students look at their results and begin analysis, draw the gel in lab reports and begin answering questions. Teacher monitors students’ progress and answers questions when needed.
- Class Discussion: What did you learn? How can this be applied to your life? Why is gel electrophoresis so important?
- Instead of buffer, Gatorade can be used as an alternative since DNA is not being used.
- Remind students, DNA “runs to red” (DNA is negatively charged so it moves toward the positive (red) electrical terminal).
- Do a test for the movement of the dyes before making solution to ensure the dyes move in the right direction (i.e. are negatively charged) and have different molecular weights (so they separate in the gel).