Days to Teach:
1 Day (60 minutes) if just doing LightCycler analysis; 1-2 Days (60-90 minutes) if also teaching background into blood type inheritance
Teaching Tips / Activity Overview:
This activity can be used after blood typing genetics have been taught and can follow an actual or simulated blood typing activity.
- Introduce the activity and do the Light Cycler Practice and Background. It may be helpful to go over an example of a LightCycler assay with the students and explain why some of the graphs show single peaks and others have double peaks based on melting point differences.
- Have students complete the ABO Homework packet.
- Answer any student questions about blood type & real-time PCR.
- Have students complete the A Mix Up In the Nursery sheet.