Days to Teach:
Two 50 minute class periods plus potential homework components.
Teaching Tips / Activity Overview:
1. Pre-lab discussion. Discuss hemoglobin structure & function. Discuss use of BLAST: 15 minutes in class.
2. Complete activity, students will analyze data on BLAST, fill in worksheets A and B, answer questions in lab protocol, and write a final summarizing paragraph: 70 minutes.
- Students should not begin the activity with all of the student materials. Distribute as follows:
- Begin with ‘Procedure’ and ‘Worksheet A’
- Distribute ‘Worksheet B’ to students only when they request it. They will be prompted to ask for it in section E of the procedure.
- As such, copy the procedure as one packet and worksheets A and B each as separate worksheets.
- If possible, the data sheet should be made available to students electronically. This will assist in their loading the amino acid sequences into BLAST.
- Emphasize that students will have to make a significant number of comparisons using BLAST, each species must be compared with every other species. If working in groups, students may wish to divide up this task.
- Likewise, it may be helpful to demonstrate the use of BLAST, especially if a smart board and/or LCD projector is available to project the BLAST tool for full class viewing.
3. Final summarizing paragraph: Homework, depending upon time requirements.
4. Wrap-up discussion, discuss results, interpretation of data, and use of bioinformatics tools: 15 minutes.
- It is also helpful to conduct a final review of the activity, generally after students have submitted their work or after it has been evaluated and returned. In this review emphasize the results and how these suggest the idea of relationship by common ancestry.