Practice Gel Electophoresis Activity: Duchenne Muscular Dystrophy

Overview & Concepts

This activity will be used to introduce the students to the proper use of pipettes, loading of dyes and reading of electrophoresis gels. This activity could be used as a paper activity without the gel lab if need be. 

Grade Level: 

Concepts Covered: 

pipetting, gel electrophoresis

Prior Knowledge Required: 

Genetics, genes, DNA

Activity Notes

Days to Teach: 

Entire Activity: 1-2 days

Materials: 

  • "Use of Micropipettors" handout
  • Micropipettors
  • "Duchenne Muscular Dystrophy Medical Case" handout
  • Gel electrophoresis equipment
  • DMD Medical Case supplies:  Carolina: 217075m 219027.  Wards 945 V 7306, 944 V 6501, Fisher Scientific AC42269-0050.  $102.70

Teaching Tips / Activity Overview: 

  1. Introduce the activity.
  2. Divide students into groups.
  3. Students practice using micropipettes with attached protocol. Be sure to monitor student understanding and micropipette use.
  4. Students perform "Duchenne Muscular Dystrophy Medical Case" activity. 

Assessment: 

  • Pre assessment: a pre-quiz will be given at the start of the biotech unit.
  • Post assessment: A test will be given at the end of the unit with gel results and questions pertaining to them. 

Resources: 

Acknowledgements: 

This lesson was written by Amy McGough.  It incorporates an activity protocol originally found in Modern Genetics for All Students (http://so.wustl.edu/teachers/curriculum/biology) and later adapted by Tara Flick and Elizabeth Rice for the Bioinformatics Professional Develop Seminar at Franklin & Marshall College. 

Academic Standards
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