Days to Teach:
About a week (depending on how much time is spent in lab and how much is assigned for homework)
Teaching Tips / Activity Overview:
- Introduce the activity and hand out the student worksheet, A Guide to Bioengineering.
- Guide students as they complete the sections in order. In some cases students will work independently or in small groups, while at other times the whole class will participate in a lab. The activity sequence is:
- Day 1: DNA Extraction questions and lab
- Homework: Complete questions about DNA fingerprinting (prior to lab)
- Day 2: DNA Fingerprinting Activity
- Homework: Complete questions about Restriction Enzymes and Gel Electrophoresis
- Day 3 and Part of Day 4: PCR
- Homework: Complete questions about PCR
- Day 4 and Day 5: Using BLAST to compare sequences
Please note the following:
- This lab is designed for an accelerated biology class, but can be adapted for an academic level class.
- The labs are not neccessary to complete. If lab supplies can not be obtained, students can simply use the online activities, diagrams, and videos to get the information they need. The labs are added to help students visualize what is happening.
- There are two different DNA extraction labs attached. One lab is straight-forward, and students simply extract DNA from different living tissues using a step-by-step procedure. The other lab attached asks students to design a DNA extraction protocol and explain what results they may get based on their understanding of DNA extractions.
- Use the DNA Fingerprinting Pictures with the Self-Guided Notes document.
- Assign the questions for homework so there is more time in class to complete the labs.
- I have not attached an offical gel electrophoresis lab. Any lab can be done here with dyes or a DNA crime solving kit.
- I have not attached an offical PCR lab. I have attached, however, a protocol that works well when trying to amplify the 16S-23S intergenic region in bacteria.